**ete4（Environment for Tree Exploration v4）**是生物信息学领域核心的Python库，专注于系统发育树（进化树）的构建、操作、可视化及分析，广泛应用于进化生物学、生态学、流行病学、基因组学等研究场景。
windows10 pip安装ete3可以，安装ete4就不行，报错信息：
pip install ete4 Looking in indexes: https://pypi.tuna.tsinghua.edu.cn/simple Collecting ete4 Using cached https://pypi.tuna.tsinghua.edu.cn/packages/fa/53/0ceedd63e4c872f668188a5de786a00b6b8854cb285a3dc69cd107c62038/ete4-4.4.0.tar.gz (1.4 MB) Installing build dependencies ... done Getting requirements to build wheel ... error error: subprocess-exited-with-error × Getting requirements to build wheel did not run successfully. │ exit code: 1 ╰─> [34 lines of output] Error compiling Cython file: ------------------------------------------------------------ ... """ ^ ------------------------------------------------------------ ete4\core\operations.pyx:1:0: 'ete4\core\operations' is not a valid module name Compiling ete4\core\operations.pyx because it changed. Compiling ete4\core\tree.pyx because it changed. Compiling ete4\parser\newick.pyx because it changed. [1/3] Cythonizing ete4\core\operations.pyx Traceback (most recent call last): File "G:\comatespec\lingjingchong\.venv\Lib\site-packages\pip\_vendor\pyproject_hooks\_in_process\_in_process.py", line 389, in <module> main() File "G:\comatespec\lingjingchong\.venv\Lib\site-packages\pip\_vendor\pyproject_hooks\_in_process\_in_process.py", line 373, in main json_out["return_val"] = hook(**hook_input["kwargs"]) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "G:\comatespec\lingjingchong\.venv\Lib\site-packages\pip\_vendor\pyproject_hooks\_in_process\_in_process.py", line 143, in get_requires_for_build_wheel return hook(config_settings) ^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\Admin\AppData\Local\Temp\pip-build-env-3y9j6ica\overlay\Lib\site-packages\setuptools\build_meta.py", line 333, in get_requires_for_build_wheel return self._get_build_requires(config_settings, requirements=[]) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\Admin\AppData\Local\Temp\pip-build-env-3y9j6ica\overlay\Lib\site-packages\setuptools\build_meta.py", line 301, in _get_build_requires self.run_setup() File "C:\Users\Admin\AppData\Local\Temp\pip-build-env-3y9j6ica\overlay\Lib\site-packages\setuptools\build_meta.py", line 317, in run_setup exec(code, locals()) File "<string>", line 29, in <module> File "C:\Users\Admin\AppData\Local\Temp\pip-build-env-3y9j6ica\overlay\Lib\site-packages\Cython\Build\Dependencies.py", line 1153, in cythonize cythonize_one(*args) File "C:\Users\Admin\AppData\Local\Temp\pip-build-env-3y9j6ica\overlay\Lib\site-packages\Cython\Build\Dependencies.py", line 1297, in cythonize_one raise CompileError(None, pyx_file) Cython.Compiler.Errors.CompileError: ete4\core\operations.pyx [end of output] note: This error originates from a subprocess, and is likely not a problem with pip. ERROR: Failed to build 'ete4' when getting requirements to build wheelAI教给个方法，使用预编译版本安装，一次就过了！
pip install --only-binary=ete4 ete4输出信息
(.venv) PS G:\traework\medai\acnes_amp_design> pip install --only-binary=ete4 ete4 Looking in indexes: https://pypi.tuna.tsinghua.edu.cn/simple Collecting ete4 Using cached https://pypi.tuna.tsinghua.edu.cn/packages/f7/5a/af998ea73723e666e2b339694221ca24bcce5cae3913bed261752adecd02/ete4-4.0.0-py3-none-any.whl (1.6 MB) Collecting bottle (from ete4) Using cached https://pypi.tuna.tsinghua.edu.cn/packages/83/f6/b55ec74cfe68c6584163faa311503c20b0da4c09883a41e8e00d6726c954/bottle-0.13.4-py2.py3-none-any.whl (103 kB) Collecting brotli (from ete4) Downloading https://pypi.tuna.tsinghua.edu.cn/packages/1c/4e/bc1dcac9498859d5e353c9b153627a3752868a9d5f05ce8dedd81a2354ab/brotli-1.2.0-cp312-cp312-win_amd64.whl (369 kB) Requirement already satisfied: numpy in G:\comatespec\lingjingchong\.venv\Lib\site-packages (from ete4) (1.26.4) Requirement already satisfied: scipy in G:\comatespec\lingjingchong\.venv\Lib\site-packages (from ete4) (1.16.3) Requirement already satisfied: requests in G:\comatespec\lingjingchong\.venv\Lib\site-packages (from ete4) (2.31.0) Requirement already satisfied: charset-normalizer<4,>=2 in G:\comatespec\lingjingchong\.venv\Lib\site-packages (from requests->ete4) (3.4.4) Requirement already satisfied: idna<4,>=2.5 in G:\comatespec\lingjingchong\.venv\Lib\site-packages (from requests->ete4) (3.11) Requirement already satisfied: urllib3<3,>=1.21.1 in G:\comatespec\lingjingchong\.venv\Lib\site-packages (from requests->ete4) (2.6.3) Requirement already satisfied: certifi>=2017.4.17 in G:\comatespec\lingjingchong\.venv\Lib\site-packages (from requests->ete4) (2025.11.12) Installing collected packages: brotli, bottle, ete4 Successfully installed bottle-0.13.4 brotli-1.2.0 ete4-4.0.0问题解决！
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